Sensitive and specific detection of Salmonid alphavirus using real-time PCR (TaqMan®)
نویسندگان
چکیده
منابع مشابه
Development of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملraPid deteCtion of total and PatHogeniC VIBRIo pARAHAemoLyTICus Using real-time PCr witH taqman® flUoresCent Probes
Vibrio parahaemolyticus is found throughout the marine environment and is a major cause of foodborne illness around the world. Foodborne illnesses due to V. parahaemolyticus infections frequently trace back to the consumption of raw or undercooked seafood. The ability to detect V. parahaemolyticus in a rapid, sensi tive, and specific manner is important to safeguard our food supply and prevent...
متن کاملApplication of Rapid and Sensitive Real Time PCR Technique in Detection of DNA Impurities in Recombinant Interferon
Background & Objective: Interferon belongs to a family of cytokines, which has the most important role in the innate immune response to virus infections. While producing recombinant interferon in biological host, some pieces of host nucleic acids remain in product. Because of limitations in previous techniques for detection of these impurities, the objective of this study is to use rapid ...
متن کاملRapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay.
Batrachochytrium dendrobatidis is a major pathogen of frogs worldwide, associated with declines in amphibian populations. Diagnosis of chytridiomycosis to date has largely relied upon histological and immunohistochemical examination of toe clips. This technique is invasive and insensitive particularly at early stages of infection when treatment may be possible. We have developed a real-time PCR...
متن کاملCoamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.
BACKGROUND DNA genotyping with mutation-specific TaqMan(R) probes (Applied Biosystems) is broadly used in detection of single-nucleotide polymorphisms but is less so for somatic mutations because of its limited selectivity for low-level mutations. We recently described coamplification at lower denaturation temperature-PCR (COLD-PCR), a method that amplifies minority alleles selectively from mix...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2006
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2005.08.012